Qualitative and Quantitative Analysis of the Phytochemicals of Azadirachta indica, Nauclea Latifolia, Vernonia ambigua, and Artemisia annua Distillates

Authors

DOI:

https://doi.org/10.56919/usci.1222.018

Keywords:

Medicinal plants, phytochemical, Quantitative Analysis, Steam distillation, Volatile constituents

Abstract

In order to treat a variety of human health issues, plants are a beneficial source for getting numerous pharmacologically active chemicals. This study aimed to qualitatively and quantitatively determine the phytochemical constituents of Artemisia annua, Azadirachta indica, Nauclea latifolia, and Vernonia ambigua. The volatile constituents of these plants were extracted using steam distillation. The phytochemical constituents were qualitatively and quantitatively determined using the standard methods. The results of the phytochemical screening show the presence of glycosides, terpenoids, alkaloids, terpenoids, saponins, and tannins. The quantitative Analysis shows that Azadirachta indica has the highest concentration of alkaloid (0.18 mg/mL), flavonoid (0.06 mg/mL), and total phenolic compounds (0.11 mg/mL). It was concluded that the distillates of the plants studied possess significant phytoconstituents. Further study needs to identify relevant compounds from the distillates for a better understanding of their mechanism of actions and activities. Furthermore, toxicology and in vivo studies should performed to deduce the safety of ingesting the distillates.

References

Abubakar, B. A., Aliyu M.M., Mikhail, S.A., Hamisu, I. and Adebayo, O.O. (2011). Phytochemical screening and antibacterial activities of vernonia ambigua, vernonia blumeoides and vernonia oocephala (asteraceae). Acta Poloniae Pharmaceutica n Drug Research. 68(1): 67-73, 067-073.qxd (ptfarm.pl

Ahmed, M., Khan, M.I., Khan, M.R., Muhammad, N., Khan, A.U., and Khan, A.R. (2013). Role of Medicinal Plants in Oxidative Stress and Cancer. OMICS Publishing Group. 2: 641. https://doi.org/10.4172/scientificreports.641

Akinwunmi, O.A., Adekeye, D.K., and Olagboye, S.A. (2020). Phytochemical quantification, In vitro antioxidant and antidiabetic potentials methanol and dichloromethane extracts of Triclisia subcordata (Oliv) leaves. Trends in Phytochemical Research Journal. 4 (1): 17-24. https://dorl.net/dor/20.1001.1.25883623.2020.4.1.4.6

Ankita, S., and Sapan P. (2018). Preliminary phytochemical screening and quantitative analysis of secondary metabolites of Mentha arvensis and Azadirachta indica. International Journal of Advanced Research and Development. 3(1):114-118. Preliminary phytochemical screening and quantitative analysis of secondary metabolites of Mentha arvensis and Azadirachta indica (multidisciplinaryjournal.net)

Atoui, K., Mansouri, A., Bosku, G., and Kefalas, P. (2005), Tea and herbal infusions: their antioxidant activity and phenolic profile, Food Chemistry. 89: 27-36. https://doi.org/10.1016/j.foodchem.2004.01.075

Broadhurst, R. B., and Jones, W. T. (1978). Analysis of condensed tannins using acidified vanillin. Journal of the Science of Food and Agriculture. 29(9): 788–794. https://doi.org/10.1002/jsfa.2740290908

Ferreira, D., Nel, R. J. J., and Bekker, R. (1999). Condensed Tannins. In Comprehensive Natural Products Chemistry. pp. 747–797. https://doi.org/10.1016/B978-0-08-091283-7.00087-4

Fleischer, T. (2003), Xylopia aethiopica A Rich: A chemical and biological Perspective. Journal of University of Science and Technology. 23:24-31. Xylopia Aethiopica (Dunal) A. Rich. : A Chemical Biological Perspective | Semantic Scholar

Franklyn, N.I., Emmanuel, I.N., Kazeem, O.K., Opeyemi, C.E., Olubanke, O.O., and Shalom, N.C. (2018). Bioactive Screening and In Vitro Antioxidant Assessment of Nauclea latifolia Leaf Decoction. AIP Conference Proceedings 1954, 030015. https://doi.org/10.1063/1.5033395

Hamoud, R., Reichling, J., and Wink, M. (2015). Synergistic antibacterial activity of the alkaloid sanguinarine with EDTA and the antibiotic streptomycin against multidrug-resistant bacteria. Journal of Pharmaceutical and Pharmacology. 67: 264–273. https://doi.org/10.1111/jphp.12326

Han, X., Shen, T., and Lou H. (2007), Dietary Polyphenols and their biological significance, International Journal of Molecular Science. 8: 950-988. https://doi.org/10.3390/i8090950

Hänsel, R., Sticher, O., and Steinegger, E. (2007). Pharmakognosie—Phytopharmazie, 8th edition, Springer Verlag, Heidelberg, Germany, Pp. 153. https://doi.org/10.1007/978-3-540-34281-6

John, B., Sulaiman, C. T., George, S., and Reddy, V. R. K. (2014). Spectrophotometric estimation of total alkaloids in selected justicia species. International Journal of Pharmacy and Pharmaceutical Sciences. 6(5): 647–648. https://innovareacademics.in/journal/ijpps/Vol6Issue5/9556.pdf

Khanal, S. (2021). Qualitative and Quantitative Phytochemical Screening of Azadirachta indica Juss. Plant Parts. International Journal of Applied Science and Biotechnology. 9(2): 122-127. https://doi.org/10.3126/ijasbt.v9i2.38050

Kumar, D., Jamwal, A., Madaam, R., and Kumar, S. (2014). Estimation of Total Phenols and Flavonoids in Selected Indian Traditional Plants. Journal of Pharmaceutical Technology, Research, and Management. 2(1) : 77–86. https://doi.org/10.15415/jptrm.2014.21006

Masoumeh, M., Zahra, M., Parastoo, Z.M., Mehdi, A., Helen, H. and Fatemeh, F. (2012). Essential Oil Composition, Total Phenol, Flavonoid, Anthocyanin and Antioxidant Activities in Different Parts of Artemisia annua L. in Two Localities (North of Iran). Journal of Medicinal Plants and By-products. 1: 13-21. https://doi.org/10.22092/jmpb.2012.108439

Nwalo, F.N., Echeta, J.O., Ude, G. D. and Itumoh, M.O. (2017). Determination of phytochemical composition and antioxidative properties of selected medicinal plant in Ikwo, Ebonyi state, FUNAI Journal of Science and Technology. 3(2): 41-55. https://fjst.org/wp-content/uploads/2015/04/Overview_Preliminary-pages-1.pdf

Omotugba, S. K., Kehinde, A. S., Ampitan, T. A. and Amusa, T. O. (2019). Phytochemical screening of selected browse species in federal college wildlife management new bussa, Niger state. Journal of Research in Forestry, Wildlife & Environment. 11(4):23-27. https://www.ajol.info/index.php/jrfwe/article/download/193018/182143

Pushpa, R., Tanuj, K.A., Pankaj, G. and Ashish T. (2015). Qualitative and quantitative phytochemical analysis of Artemisia indica Willd. Journal of Chemical and Pharmaceutical Research. 7(4):942-949. https://www.jocpr.com/articles/qualitative-and-quantitative-phytochemical-analysis-of-artemisia-indica-willd-3254.html

Ramawat, K.G., and Goyal, S. (2008). The Indian herbal drugs scenario in global perspectives. In: Ramawat, K.G., and Merillon, J.M (eds) Bioactive molecules and medicinal plants. Springer Berlin, Heidelberg. Pp. 82. https://doi.org/10.1007/978-3-540-74603-4

Schippmann, U., Leaman, D.J., and Cunningham, A.B. (2006) Plants as source of medicines: New perspectives. In: Bogers RJ, Craker LE, Lange D, eds. Medicinal and aromatic plants – Agricultural, commercial, ecological, legal, pharmacological, and social aspects. Dordrecht: Springer. Pp. 71.

Singleton, V. L., and Rossi, J. A. J. (1965). Colorimetry of Total Phenolics with Phosphomolybdic acid Reagents. American Journal of Enology and Viniculture. 16(3):144–158. https://www.ajevonline.org/content/16/3/144

Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria, pp. 191-289.

Uche, F.I., Abed, M.N., Abdullah, M.I., Drijfhout, F.P., McCullagh, J., Claridge, T.W., Richardson, A., and Li, W.W. (2017) Isochondodendrine and 2′-norcocsuline: additional alkaloids from Triclisia subcordata induce cytotoxicity and apoptosis in ovarian cancer cell lines. RSC Advances. 7(70): 44154-44161. https://doi.org/10.1039/C7RA08032H

Verpoorte R. (2009). Medicinal plants: A renewable resource for novel leads and drugs. In: Ramawat KG, ed. Herbal drugs: Ethnomedicine to modern medicine. Berlin Heidelberg: Springer-Verlag. Pp. 54

Victor, N. O. and Chidi O. (2009). Phytochemical constituents of some selected medicinal plants. African Journal of Pure and Applied Chemistry. 3 (11): 228-233. https://doi.org/10.5897/AJPAC.9000111

Wink, M. (2012). Medicinal plants: Source of anti-parasitic secondary metabolites. Molecules. 17: 12771–12791. https://doi.org/10.1007/978-3-540-79116-4_1

Wink, M., Schmeller, T., and Latz-Brüning, B. (1998). Mode of action of Allelochemical alkaloids: Interaction with neuroreceptors, DNA and other molecular targets. Journal of Chemical Ecology. 24: 1881 – 1937. https://doi.org/10.1023/A:1022315802264

Downloads

Published

2022-12-30

How to Cite

Muhammad Zara, Masanawa Abubakar Aliyu, & Abdulazeez Ridwan. (2022). Qualitative and Quantitative Analysis of the Phytochemicals of Azadirachta indica, Nauclea Latifolia, Vernonia ambigua, and Artemisia annua Distillates. UMYU Scientifica, 1(2), 147–156. https://doi.org/10.56919/usci.1222.018