Antibacterial Activities of Three (3) Selected Medicinal Plants against Diarrhea Causing Pathogens
DOI:
https://doi.org/10.56919/usci.2432.013Keywords:
Diarrhea Pathogens, plant extracts, Antibacterial activityAbstract
Study’s Excerpt/Novelty
- This study offers a comparative analysis of the antimicrobial efficacy of extracts from Acacia nilotica, Carica papaya, and Khaya senegalensis against common diarrheal pathogens, providing insights into potential alternative treatments for diarrhoea.
- Utilizing the cold maceration method and well diffusion assays, the research meticulously quantifies the inhibitory effects of each extract at varying concentrations, revealing Khaya senegalensis as the most potent.
- The findings underscore the therapeutic potential of these plant extracts, particularly in developing regions, and support their traditional use in diarrhoeal therapy, presenting a promising avenue for addressing a critical public health issue.
Full Abstract
Diarrhoea is one of the leading causes of morbidity and mortality worldwide, especially in developing countries in sub Saharan Africa and south East Asia. Extracts from Acacia nilotica,(Bagaruwa) Carica papaya (Gwanda) and Khaya senegalensis (Marke) were prepared using cold maceration method. Well diffusion method was used on Muller Hinton agar and thiosulfate citrate bile salt agar (TCBS) and suspension of the test organisms was prepared in peptone water to match 0.5 McFarland standard, each extract solution at 200, 250 and 300mg/ml, was introduced into respective wells, incubated at 37oC for 24 hours. Ciprofloxacin at 8 mg/ml, was used as control. Plant crude extracts of C. papaya yielded (19.05g), A. nilotica (18.67g) and K. senegalensis (13.94g) respectively. The extracts exhibited varying degrees of inhibition against V. cholerae, K. pneumoniae and S. enterica serovar. Khaya senegalensis had the highest significant antibacterial activity at 300mg/ml (36.00±0.81f, 19.00±0.98, 22.00±0.69) and was the most active among the three plant extracts, followed by C. papaya at 300mg/ml (29.33±1.17, 31.50±0.29, 9.00±1.15) then A. nilotica at 200mg/ml, 29.00±0.87, 12.00±0.58, 20.00±0.29. Only C. papaya showed no activity at all concentration against V. cholera. The MIC of the plant crude extracts against Vibrio cholerae, Klebsiella pneumoniae and Salmonella enterica serovar Kentucky was 0.96±0.04, 0.96±0.05 and 1.80±0.17mg/mL respectively, while MBC was 1.02±0.12, 1.04±0.29 and 1.92±0.23 mg/mL. In conclusion the three plant crude extracts of (K. senegalensis, A. nilotica and C. papaya) showed varying degrees of antimicrobial activities against the test organisms. Therefore the leaves of the plants Carica papaya, Acasia nolitica and K. senegalensis can be recommended for diarrhoeal therapy in accordance with the traditional uses.
References
Abalaka M. E., Akpor O. B., Bello A. B., Olonitola O. S., Sani A. and Osemwegei O. O. (2016). Synthesis of Silver Nano Particles (AgNPs) from Bark and Rooth of African Mahogany (Khaya senegalensis) and the Comparative Study of their Antimicrobial Properties. Asian Journal of Microbiology and Biotechnology 1(1): 25-32. https://doi.org/10.47430/ujmr.1611.007
Abdallah, M. S., Ahmed, I., & Yahaya, S. M. (2016). Antibacterial activity and phytochemical screening of leaf and stem (bark) extract of Khaya senegalensis against Diarrhoeal stool isolates. Cibtech Journal of Microbiology, 5(1), 17-24.
Agarry O.O, Olaleye M.T and Bello-Michael, C. O. (2005) Comparative antimicrobial activities of Aloe vera gel and leaf. African Journal of Biotechnology Vol. 4 (12), pp. 1413-1414,
Ahmad I.M. and Wudil A.M. (2013). Phytochemical Screening and Toxicological Studies of Aqueous Stem Bark Extract of Anogeissus leiocarpus in Rats. Asian Journal of Scientific Research 6 (4) 781-788.
Akpan, M. M., Odeomena, C. S., Nwachukwu, C. N. and Danladi, B. (2012). Antimicrobial assessment of ethanolic extract of Costus afer Leaves. Asian Journal of Plant Science and Research, 2(3), 335-341
Ali, S. A. M., Elbadwi, S. M. A., Idris, T. M., and Osman, K. M. (2011). Hepatoprotective activity of aqueous extract of Khaya senegalensis bark in rats. Journal of Medicinal Plants Research, 5(24), 5863-5866.
Ali, S. A. M., Elbadwi, S. M. A., Idris, T. M., and Osman, K. M. (2017). Hepatoprotective activity of aqueous extract of Khaya senegalensis bark in rats. Journal of Medicinal Plants Research, 5(24), 5863-5866.
Amadou, I., Soulé, M., and Salé, A. (2020). An overview on the importance of Acacia nilotica (L.) willd. ex del.: A review. Asian Journal of Resarch in Agriculture and. Forestry, 5, 12-18. https://doi.org/10.9734/ajraf/2020/v5i330085
AOAC Association of Official Analytical Chemists, AOAC (2010). Official methods of analysis. Association of Official Analytical Chemists, 15th Edition Washington D.C., USA. 184pp
Chomini, S. M., Joshua, V. I., Ukwadi, M., Sadiku, Y., Popoola, S. A., Imoh, J. A., and Usman, S. A. (2021) Phytochemical Screening and Antibacterial Activities of Anogeissus leiocarpa (DC.) Gull. and Perr. Leaf Extracts against Escherichia coli (T. Escherichia) and Salmonella typhimurium (Loeffler). Australian Journal of Science and Technology, 5(2), 527-532.
Cohen, A. L., Platts-Mills, J. A., Nakamura, T., Operario, D. J., Antoni, S., Mwenda, J. M. & Serhan, F. (2022). Aetiology and incidence of diarrhoea requiring hospitalisation in children under 5 years of age in 28 low-income and middle-income countries: findings from the Global Pediatric Diarrhea Surveillance network. BMJ Global Health, 7(9), e009548. https://doi.org/10.1136/bmjgh-2022-009548
Doughari, J. H. (2012). Phytochemicals: extraction methods, basic structures and mode of action as potential chemotherapeutic agents (pp. 1-33). Rijeka, Croatia: INTECH Open Access Publisher.
EVBUOMWAN, L; CHUKWUKA, EP; OBAZENU, EI; ILEVBARE, L (2018) Antibacterial Activity of Vernonia amygdalina Leaf Extracts against Multidrug Resistant Bacterial Isolates. J. Appl. Sci. Environ. Manage 22 (1) 17-21. https://doi.org/10.4314/jasem.v22i1.4
Farouk, M.A, Musa, A., and Yakasai I.A. (2016). Determination of Cadmium and Lead Levels In Meat, Liver And Kidney Of Cow And Goat Marketed In Zaria, Nigeria. Bayero Journal of Pure and Applied Sciences, 9(1) 180 – 184. https://doi.org/10.4314/bajopas.v9i1.28
Galalain, M. A., Habib, A. A., Tukur, S., Bashir, A. R., and Abdu, K. (2019). Antifungal efficacy of Anogeissus leiocarpus DC against Aspergillus niger and Rhizoctonia spp. Fudma Journal of Sciences-ISSN: 2616-1370, 3(2), 179-184.
Ibrahim, M. A., Musa, A. M., Aliyu, A. B., Mayaki, H. S., Gideon, A., and Islam, M. S. (2013). Phenolics-rich fraction of Khaya senegalensis stem bark: antitrypanosomal activity and amelioration of some parasite-induced pathological changes. Pharmaceutical Biology, 51(7), 906-913. https://doi.org/10.3109/13880209.2013.771191
Jafari, F., Hamidian, M., Rezadehbashi, M., Doyle, M., Salmanzadeh-ahrabi, S., Derakhshan, F. & Zali, M. R. (2008). Prevalence and antimicrobial resistance of diarrheagenic Escherichia coli and Shigella species associated with acute diarrhea in Tehran, Iran. Canadian Journal of Infectious diseases and Medical Microbiology, 19(3), e56-e62. https://doi.org/10.1155/2009/341275
Kamboj, V. P. (2000). Herbal medicine. Current science, 78(1), 35-39.
Kuta F. A., Tsado D. J., Garba S. A. and Saidu N. A. (2015). Antibacterial Activity of the Leaf and Stem bark crude extract of Khaya senegalensis. Medicinal and Aromatic Plant Research Journal Vol. 3(1) pp 9-15
Mann, A., Yusuf, A. and Daniyan, S. (2014). TLC analysis and bioactivity screening of the stem bark extract of Anogeissus leiocarpus against multi-resistant Staphylococcus aureus and quantification of its phytoconstituents. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5, (187-203).
Mohammed A., K.Ghoneim, M. Amer, A. Al-Daly, F. Khadrawy and M.A. Mahmoud (2014). Transaminase perturbation in certain tissues of Schistocerca gregaria(Forskal) (Orthoptera: Acrididae) by Punica granatum Linn. (Lythraceae) extracts
Mohammed, S. and Tamiru, D. (2014). The burden of diarrheal diseases among children under five years of age in Arba Minch district, Southern Ethiopia, and associated risk factors: A cross‑sectional study. International Scholarly Research Notices 2014, 654901. https://doi.org/10.1155/2014/654901
Muhammad, B., Sadiq, I., Joel, T., Tay, Z., Aye, C. and Anil, K.A. (2015). Antibacterial Activities and Possible Modes of Action of Acacia nilotica (L.) Del. against Multidrug-Resistant Escherichia coli and Salmonella. https://doi.org/10.3390/molecules 22010047.9. https://doi.org/10.3390/molecules
Musah, S. (2019). Antibacterial activity of matured leaf extract of Poliostigma thoningii against E. coli and S. enterica Typhi at various concentrations. African Journal of Biotechnology, 18(12), 399-406. https://doi.org/10.5897/AJB2019.16832
Namadina, M. M., Mukhtar, A. U., Karaye, S. I., Musa, F. M., Bah, I. H., Maitama, F. Y. (2021) Biological Activity and Phytochemical Constituents of Anogeissus leiocarpus Stem Bark Extracts. Bayero Journal of Pure and Applied Sciences, 14(1), 85- 94. https://doi.org/10.4314/bajopas.v14i1.12
NCCLS (2002) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals, Approved Standard. 2nd Edition, NCCLS Document M31-A2 22(6), Clinical and Laboratory Standards Institute, Wayne. https://doi.org/10.1016/j.fct.2006.01.006
Nikom Srikacha and Khakhanang Ratananikom (2020). Antibacterial activity of plant extracts in different solvents against pathogenic bacteria: An in vitro experiment. Journal of Acute Disease. ; 9(5): 223-226. https://doi.org/10.4103/2221-6189.291288
Ramos Morales, E. ; Molina Alcaide, E. ; Sanz Sampelayo, M. R. S., (2008). Milk production of dairy goats fed diets with different legume seeds: Effects of amino acid composition of the rumen undegradable protein fraction. J. Sci. Food Agric., 88 (13): 2340-2349. https://doi.org/10.1002/jsfa.3355
Seddik S. Bun , H. Bun , D. Guédon, C. Rosier , E. Ollivier (2005). Effect of green tea extracts on liver functions in Wistar rats Journal of Food and Chemical Toxicology. Volume 44, Issue 7, July 2006, Pages 1108-1113
Shinde, A., Kolhe, D., Hase, D. P., and Chavan, M. J. (2020). A brief introduction about Carica papaya linn. World Journal of Pharmaceutical Research. 9(12), 237-244.
Ugoh Sylvanus Chukwudi * Oluwabunmi Olaitan Agarry, and Samuel Alimi Garba (2014). tudies on the antibacterial activity of Khaya senegalensis [(Desr.) A. Juss)] stem bark extract on Salmonella enterica subsp. enterica serovar Typhi [(ex Kauffmann and Edwards) Le Minor and Popoff]. Asian Pac J Trop Biomed. 79-S283. https://doi.org/10.12980/APJTB.4.2014C636
Usman, A. H., Ali, T., & Danjani, A. G. (2020). Phytochemical and antimicrobial studies of stem-bark extracts of Anogeissus leiocarpus found in Dutsin-ma, katsina-nigeria. Fudma Journal of Sciences, 4(2), 156-167. https://doi.org/10.33003/fjs-2020-0402-209
WHO Cholera, vaccines: WHO, position paper - August 2017, Epidemiological Record 25 August 2017, No 34, 2017, 92, 477-500.
World Health Organization . Cholera, (2015). Weekly Epidemiological Record. 96:445-54.
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