In vitro Inhibitory Potential of Trichoderma Species on Fusarium oxysporum f.sp vasinfectum the Causal Organism of vascular wilt of Cotton (Gossypium hirsutum l.) in the Nigerian Sudan Savanna
DOI:
https://doi.org/10.56919/usci.1122.016Keywords:
in vitro, Fusarium oxysporum f.sp. vasinfectum, Trichoderma sp., Cotton, Vascular wiltAbstract
The purpose of this study was to determine the inhibitory potential of three species of Trichoderma namely; Trichoderma asperelum, Trichoderma viride and Trichoderma harzianum on Fusarium oxysporum f.sp vasinfectum (fov), the causal organism of vascular wilt in cotton. The experiment consisted of dual culture incubated at 250C + 10C on PDA, for 9 days. During the experiment, the three trichoderma species were obtained from the soil while the fusarium oxysporum isolate was obtained from IAR, Zaria, Nigeria. Antagonistic activity testing was determined using percentage inhibition of Fusarium oxysporum radial growth. The study's results revealed that all the three Trichoderma species tested in this experiment had significantly inhibited the mycelial growth of fov at different degrees compared with untreated control. The percentage inhibition ranged from 54% for Trichoderma. asperelum, 62.8% by Trichoderma viride to the highest being 75.6% and a mean of 45.50% due to Trichoderma harzianum. These results showed that Trichoderma harzianum was the most effective followed by Trichoderma viride and lastly Trichoderma asperellum. This suggest that there are some similarities between the three isolates of Trichoderma as all the three species could inhibit the growth of Fusarium oxysporum f.sp vasinfectum but the best to be used as bio-control agent for vascular wilt of cotton caused by fov was Trichoderma harzianum pending further research.
References
Abdul U.B (2016). Profitability and production efficiency in cotton production in North west Nigeria.. Department of agricultural Economics and Rural Sociology Ahmadu Bello University Zaria. Unpublished PhD Thesis. Pp 10-127
Adeniji B (2007). Constrains to improve cotton production in Katsina state Nigeria. Journal of Applied Sciences. 12; 1647-1651. [Crossref] https://doi.org/10.3923/jas.2007.1647.1651
https://doi.org/10.3923/jas.2007.397.401
Akarami M., Golzorry H.,Ahmadzadah M (2011). Evaluation of different combination of Trichoderma species for controlling Fusarium rot of Lentil. African Journal of Biotechnology. 14; 2653-2658. [Crossref] https://doi.org/10.5897/AJB10.1274
Arya A.,Sharma R., Sharma G., Chandra B., Negi A., and Mishra B (2017). Evaluation of fungal and bacterial antagonists for managing phytopathogen Fusarium moniliforme var, subglutinans Sheldon, causing pokkahboeng disease of sugar cane. Journal of Biological control. 31; 217-222. [Crossref]
https://doi.org/10.18311/jbc/2017/15456
Brubaker, C.L.; Seelanan, T, Stewart, J.M.; Craven, L.A; Wendel, J.F (1999) Molecular Systematic of Australian Gossypium section Grandicalyx (Malvaceae). Systematic Botany 24; PP1 83-208. [Crossref] https://doi.org/10.2307/2419548
Dhodi A., Sunita J., Wegmare P (2018). Biopotential of isolates of Trichoderma against Fusarium oxysporum vasinfectum (causing wilt disease of cotton). Journal of Scientific Development in Agriculture and Tecnology in India. 1(13): 68-70.
Dolatobadi K. Goltapeh E, Mohammad N, Rabiey N, Rohani N and Verma A (2012). Biopotential of root endophytic fungi and Trichoderma spp. Against Fusarium wilt of Lentil under in vitro and Green house condition. Journal of Agricultural Technology. 14:. 407-420.
Finelib. (2017). Cotton producing states in Nigeria and it's cultivation process. www. Finelib. Com 233-238
Flegel, T. W. (1980). Semipermanent microscope slides of microfungi using sticky tape technique, Canadian Journal of Microbiology. 26: 551-553. [Crossref] https://doi.org/10.1139/m80-095
Fogliano V, Ballio A, Gallo M, Woo S and Lorito M (2002). Pseudomonas lepsidopeptide sand fungal cell wall degrading enzymes act synergistically in Biological control, .Mol plant- mic inter. 15: 323-333. [Crossref] https://doi.org/10.1094/MPMI.2002.15.4.323
GenStat Release 7.2 DE (PC/Windows XP) Copyright 2014, Lawes Agricultural Trust (Rothamsted Experimental Station).
Kannangara S. Dharmarathna and Jayarathn S (2017) "Isolation and characterization of Trichoderma species as potential biocontrol against Ceratolystis paradoxa" Journal of Agricultural Science.1(12):51-62. [Crossref]
https://doi.org/10.4038/jas.v12i1.8206
Kareem, K., Ugoji, E and Abaobu O (2016) "biocontrolof Fusarium longibrachiatum NGJ167 (Rifu)" British Microbiology research Journal. 5(16) 1-11. [Crossref] https://doi.org/10.9734/BMRJ/2016/28208
Khadi B.M, SanthyV andYadav M.S (2010). Cotton an introduction. 00848. Pp 1-6. [Crossref]
https://doi.org/10.1007/978-3-642-04796-1_1
Kornerup A and Wancher J.H (1978). Matheun hand book of color. London publishers. Pp 248
Kshirsagar C and Todkar L (2005). Assessment of bioagent isolated from wilt suppressive soils against Fusarium oxysporum f.sp vasinfectum. Journal cotton research and development..1(19):109-111.
Kucuk, C and Kivanc, M.(2003). Isolation of Trichoderma spp. and determination of their antifungal, biochemical and physiological features. Turkish Journal of Biology 123-127
Kutama, A.S., Abubakar, M. M., Kabiru, S. and Maharaz, A. (2016): Survey of fusarium wilt of garden egg (Solanum melongena) at Imawa village of Kura Local Govt., Kano State, Nigeria. International Journal of Innovative science, Engineering and Technology, 3(1):95-99.
Kutama, A. S., Umar, S., Abdullahi, T. and Hadiza, M. S. B. (2013). Inhibition of FusariumoxysporumF. spLycopersici, the Causal Organism of Fusarium Wilt in Tomato by Azadirachta indica and Anogeissus leiocarpus Leaf Extracts. International Journal of Applied Research and Technology. 2(9): 120 - 126.
Kutama, A. S. and Aliyu, B.S. (2007) Comparative Study on the Effect of Soil Composition on the Vegetative Growth Characters of Cotton (Gossypium hirsutum L.) In Kano and Katsina States, Nigeria. Biological and Environmental Sciences Journal for the Tropics, 4(1): 189-190.
Manjur, M. and Afiya, H. (2019). Identification and isolation of Trichoderma spp - Their significance in Agriculture, Human health, Industrial and Environmental application. Intechopen. 1-12. [Crossref] https://doi.org/10.5772/intechopen.83528
Naher, L., Yusuf, U and Ismail A. (2014). "Trichderma species as biological agent for sustainable management of plant diseases" Pakistan journal of Botany.46(4):1489-1493.
Nigerian Finder (2017). Cotton farming in Nigeria step by step guide. Nigerian finder. Com.
Oman M and Zeilinger S (2010). How a mycoparasite employ G-protein signaling using the example of Trichoderma. Journal of signal Trans. Pp 1-8. [Crossref] https://doi.org/10.1155/2010/123126
Queensland (2018). "Fusarium wilt of cotton" the state of Queensland (Department of Agriculture and fisheries.
Raza W, Faheem M, Yousaf S, Rajer F. U. and Yameen, M. (2013). Volatile and non volatile produced by TrichodermaharzianumSQR-T037 supressed the growth of Fusarium oxysporum f.sp neveum. Sci let 1(1). 21-24.
Sangayomi TE (2004). Post harvest fungal deterioration of Yam (Dioscorearotundata) and its conrol. PhD theses IITA Ibadan Nigeria, 179pp.
Savazzini, F and Longa(2009). Impact of biocontrol agents Trichdermaatrovideon soil microbial communities of Vineyard in northern Italy." BiolBiochem. 41:1457-1465. [Crossref]
https://doi.org/10.1016/j.soilbio.2009.03.027
Shama p., Shama M and Shanmugan V (2014). State of Trichoderma Research in India. A review, Indian journal of phytopathology. 14( 68): 1-19.
Tonoh K.,Bolou B., Souleyman C., Kuakun, K and Douda, k. (2015). "biopesticides of some Trichodermaspecies against growth of Fusariumoxysporumf.spvasinfectum, causal agent of cotton wilt. British microbiology research Journal.10(5) 1-11. [Crossref] https://doi.org/10.9734/BMRJ/2015/20518
Vinale F., Sivasithamparam K., Ghisalbeti E., Mara R.,Woo S and Lorito M (2008). Trichodermaplant pathogen interaction. Journal of soil biology Biochem. 40(10): 1-10. [Crossref]
https://doi.org/10.1016/j.soilbio.2007.07.002
Waghunde R., Shelake M., and para M (2016). Trichoderma: a significant fungus for Agriculture and Environment. African Journal of Agricultural Research. A review. 22, 1952-1965. [Crossref] https://doi.org/10.5897/AJAR2015.10584
Wang B., Brubakar C., Tale, W., wood M., Marathon B., and Burden J (2006). "Genetic variation and population structure ofFusariumoxysporumf.spvasinfectum, in Australian cotton." Journal of Plant Pathology. (55)746-755. [Crossref]
https://doi.org/10.1111/j.1365-3059.2006.01445.x
Watanabe T (2002). Pictorial Atlas of soil and seed fungi: morphology of cultured fungi and key to species second edition. CRS press. [Crossref] https://doi.org/10.1201/9781420040821
Yahaya, N., Kutama, A. S., Karamba, H. and Ahmad, M. K. (2013): Mycorrhiza fungi as biocontrol agent of plant pathogens: A Review. Biological and Environmental Sciences Journal for the Tropics (3):47-50.
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