Antibacterial activity of Moringa oleifera (lam) seed against Streptococcus pneumoniae and Staphylococcus aureus
DOI:
https://doi.org/10.56919/usci.1122.001Keywords:
phytochemical analyses, Moringa oleifera seed, Minimum Inhibitory Concentration, Antibacterial activity, Agar-well diffusionAbstract
Medicinal plants represent a rich source from which antimicrobial agents may be obtained and are used medicinally in different countries as source of many potent and powerful drugs. Moringa oleifera Lam. Is found to be very useful tree in tropical countries. All parts of the tree are used in different healing procedures for different diseases. The antibacterial activity of Moringa oleifera (lam) seed against Streptococcus pneumoniae and Staphylococcus aureus was investigated in this study. The maceration method was used to extract the plant seed material. Phytochemical analyses of the seeds in ethanol solvents were also performed. The antibacterial effect of the extracts on the test organisms was evaluated using the agar-well diffusion method. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the seed crude extract were evaluated to determine the static and cidal effect of the seed extract against the test bacteria. The results showed that the extract contained steroids, terpenoids, phenol, alkaloids, and tannins but no saponins, flavonoids, and anthraquinones. The antibacterial activity was exceptional against both bacteria with zones of inhibition of 17.33±0.58mm at 200 mg/ml, 16.33±0.34mm at 100 mg/ml, and 15.33±0.11mm at 50 mg/ml for Staphylococcus aureus, and 13.67±0.21mm at 200mg/ml 12.67±0.22mm at 100mg/ml, 11.67±0.05mm at 50mg/ml for Streptococcus pneumoniae. Because Moringa oleifera seeds were found to have significant antibacterial activity against the microorganisms tested, the findings of this study support Moringa oleifera's traditional use and can be recommended for use as an antimicrobial agent.
References
Center for Disease Control (CDC) (2008). Progress in introduction of pneumococcal conjugate vaccine--worldwide, 2000-2008. MMWR. Morbidity and Mortality Weekly Report, 57(42), 1148–1151.
Alzohairy, A. M. (2017). Antimicrobial activity of methanolic extracts of leaves and seeds of three moringa species grown in egypt against some antimicrobial activity of methanolic extracts of leaves and seeds of three moringa species grown in egypt against some human pathogens. January.
Anwar, F., & Rashid, U. (2007). Physico-chemical characteristics of Moringa oleifera seeds and seed oil from a wild provenance of Pakistan. Pakistan Journal of Botany, 39(5), 1443–1453.
Banso, A., & Ayodele, O. P. (2001). Activities of extracts of Garcina kola against Escherichia coli and Aspergillus niger. Journal of Applied Science and Management, 5, 58–65.
Bukar, A., Uba, A., & Oyeyi, T. (2010). Antimicrobial profile of Moringa oleifera Lam. extracts against some food–borne microorganisms. Bayero Journal of Pure and Applied Sciences, 3(1). https://doi.org/10.4314/bajopas.v3i1.58706
Dodiya, B., Amin, B., Kamlaben, S., & Patel, P. (2015). Antibacterial activity and phytochemical screening of different parts of Moringa oleifera against selected gram positive and gram negative bacteria. J. Pharm. Chem. Biol. Sci, 3, 421–425.
Fahey, J. (2005). Moringa oleifera: A Review of The Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees for Life Journal, 1(5), 1–15.
Fowoyo, P., & Oladoja, E. (2015). Phytochemical Screening, Nutritional Composition and Antimicrobial Activity of Moringa Oleifera Seed and Leaf Extract against Selected Gastrointestinal Pathogens. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS, 10(6), 116–124. https://doi.org/10.9790/3008-1062116124
Harristoy, X., Fahey, J., Scholtus, I., & Lozniewski, A. (2005). Evaluation of antimicrobial effects of several isothiocyanates on. Helicobacter Pylori. Planta Medica, 71, 326–330. https://doi.org/10.1055/s-2005-864098
Henriques-normark, B., & Tuomanen, E. I. (2013). The Pneumococcus : Epidemiology ,. Cold Spring Harbor Laboratory Perspectives in Medicine, 3(10), 1–16. https://doi.org/10.1101/cshperspect.a010215
Jabeen, R., Shahid, M., Jamil, A., & Ashraf, M. (2008). Microscopic evaluation of the antimicrobial activity of seed extracts of Moringa oleifera. Pak J Bot, 40(4), 1349–1358.
Jamil, A., Shahid, M., Khan, M. M., & Ashraf, M. (2007). Screening of some medicinal plants for isolation of antifungal proteins and peptides. Pakistan Journal of Botany (Pakistan).
Jonsson, S., Musher, D. M., Chapman, A., Goree, A., & Lawrence, E. C. (1985). Phagocytosis and Killing of Common Bacterial Pathogens of the Lung by Human Alveolar Macrophages. The Journal of Infectious Diseases, 152(1), 4–13. https://doi.org/10.1093/infdis/152.1.4
Nepolean, P., Anitha, J., & Emilin, R. R. (2009). Isolation, analysis and identification of phytochemicals of antimicrobial activity of Moringa oleifera Lam. Current Biotica, 3(1), 33–37.
Nwosu, M. O., & Okafor, J. I. (1995). Preliminary studies of the antifungal activities of some medicinal plants against Basidiobolus and some other pathogenic fungi: Vorläufige Studien zur antimyzetischen Aktivität einiger offizineller Pflanzen auf Basidiobolus und andere pathogene Pilze. Mycoses, 38(5‐6), 191–195.
Paulista, U. E., & Brasil, M. F. (2021). Phytochemical and antibacterial investigation of Moringa oleifera seed : experimental and computational approaches. https://doi.org/10.26850/1678-4618eqj.v46.2.2021.p17-25
Thilza, I. B., Sanni, S., Isah, Z. A., Sanni, F. S., Talle, M., & Joseph, M. B. (2010). In vitro Antimicrobial activity of water extract of Moringa oleifera leaf stalk on bacteria normally implicated in eye diseases. Academia Arena, 2(6), 80–82.
Von Eiff, C., Becker, K., Machka, K., Stammer, H., & Peters, G. (2001). Nasal carriage as a source of Staphylococcus aureus bacteremia. New England Journal of Medicine, 344(1), 11–16.
Wertheim, H. F. L., Vos, M. C., Ott, A., Van Belkum, A., Voss, A., Kluytmans, J. A. J. W., Van Keulen, P. H. J., Vandenbroucke-Grauls, C. M. J. E., Meester, M. H. M., & Verbrugh, H. A. (2004). Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet, 364(9435), 703–705. https://doi.org/10.1016/S0140-6736(04)16897-9
Winn, W. C., Allen, S., & Janda, W. (2006). Koneman’s color atlas and textbook of diagnostic microbiology: Lippincott Williams & wilkins. Philadelphia, PA.
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