Phytochemical screening and antioxidant activities of the bark of Lawsonia inermis (Henna) grown in Dekina, Kogi, Nigeria
DOI:
https://doi.org/10.56919/usci.1122.035Keywords:
Phytochemical, screening, lawsonia inermis, antioxidantAbstract
-
Severe health conditions can result from lack of knowledge on the bioactive constituents of medicinal plants, as it the main stay of treatment and prevention of diseases in our locality. Hence, the need to examine the therapeutic active ingredients responsible for plants medicinal ability for better health management. This study analyses the phytochemical constitutes and anti – oxidant activity of the bark of lawsonia inermis. Lawsonia inermis bark was collected, cut into pieces and dried at room temperature. The dried sample was pulverized into fine particle. 200g of the pulverized sample was weighed into a 750mL container and was added 300mL absolute ethanol, then allowed to stand for extraction. The solute obtained by evaporating the ethanol was screened for the presence of phyto – components and the result shows alkaloids, glycosides, saponins, phenols, flavonoids, proteins and amino acid as bioactive substances. Also, lawsonia inermis bark displayed an efficient free radical scavenging potential as the anti – oxidant ability showed the percentage KMnO4 radical scavenging activities of 41.4% which shows close competence with the standard ascorbic acid; 47.9% at EC50; This indicate lawsonia inermis bark to exhibit a good potential as antioxidant and could scavenge free radical from the body. The presence of the bioactive substances signifies its usefulness in therapeutic health as agent that could inhibit diseases causing several ailments and as well be a good source for health management control.
References
Arirudran, V., Krishnan, U. S. and Mahadeva, R. (2019). Preliminary phytochemical analysis and isolation and identification of active principle component of petroleum extract from leaves of lawsonia inermis L. Research journal of pharmacy and technology, 12(8), 3629 - 3636. [Crossref]
https://doi.org/10.5958/0974-360X.2019.00619.X
Chetty, K. M. (2008). Flowering of Chittor. Edn 1. Andhra Pradesh. Pp132
Debabriya, D., Dipu, S., Rajat, B., Suchita, S., Bidisha, M., Sayak, G. and Debleenn, R. (2020). Insight into the phytochemical potential of lawsonia inermis for future small molecule based therapeutic application. International research journal. 11(1), 1 - 7. [Crossref]
Deepak, M. K., Srendra, S. K., Mahabaleshwar, V. H. and Hanhong, B. (2015). Significance of antioxidant potential of plants and its relevance to therapeutic application. Int. J. Biol Sci, 11(8), 982 - 991. [Crossref]
https://doi.org/10.7150/ijbs.12096
Del Rio, L. A. (2015). ROS and RNS in plant physiology: an overview. J. Exp. Bot. 66, 22827 - 2837. [Crossref] https://doi.org/10.1093/jxb/erv099
Dharmesh, G., Darpini, S. P., Kishor, K. D., Santosh, S and Timir, B. P. (2020). Phytochemical and antimicrobial activity methanolic extract of lawsonis inermis L bark. Current issues in pharmacy and medical sciences. 33(3). 132 - 138. [Crossref] https://doi.org/10.2478/cipms-2020-0024
Farzana, K., Mohammed - Awais, F., Hafiz - Muhammad, U. F., Abdul - Rahim, S., Atif - Ali, K. K., Chul - Woong, K., Muhamma, H. M., Gaber- El- saber, B., Kyung, H.C. and Abdul, S.M. (2021). Phytochemical Investigation, Antimicrobial, Antioxidant and Anticancer Activities of Acer Cappad Ocicum Gid, doi.org/10.3390.life.11070656.
Gabriel, A. A., Olukayode, Y. O. and Taiwo, O. E. (2017). Quantitations of phytochemicals and biocide actions of lawsonia inermis Linn extract against wood termites and fungi. International Biodeterioration and Biodegradation. 116. 155 - 162. [Crossref] https://doi.org/10.1016/j.ibiod.2016.10.026
Gagandeep, C., Sandeep, G. and Priyanka, P. (2010). Lawwsonia inermis Linnaeus: a phytophamacological Review. International journal of pharmaceutical sciences and drug Research. 2 (2). 91 - 98
Hassan, W., Noreen, H., Rehman, S., Gul, S., Kamal, M. A., Kamdem, J. P. (2017). Oxidative stress and antioxidant potential of one hundred medicinal plants. Curr.Top.Med.chem, 17, 1336 - 1370. [Crossref]
https://doi.org/10.2174/1568026617666170102125648
Iram, G., Maria, S., Muhammed, S. A. and Muhammed, A. A. (2013). Phytochemical, toxicological and antmicrobial evaluation of lawsonia inermis extract against clinical isolation of pathogenic bacteria. Ann. Clin microbial antimicrobs. 12. 36. [Crossref]
https://doi.org/10.1186/1476-0711-12-36
Isaac, K. A., Emmanuel, O., Abraham, Y. M., Francis, M. S., Francis, A. and Linda, M. S. (2016). Development and validation of Radical Scavenging antioxidant assay using potassium permanganate. J. Scientific & Innov. Res, 5(2), 36-42. [Crossref]
https://doi.org/10.31254/jsir.2016.5202
Isah, A. A., Abuh, L. O., Omachi, A. A, Usman, A. and Azeez, A. A. (2020). proximate analysis, Phytochemical screening, Antioxidant activities and Potassium/Sodium determination of yellow Ipomoea batata grown in Ankpa, Kogi state Nigeria. FUW trends in science & technology journal, 5(1), 226 - 230.
Jiraporn, L., Churdsak, J., Kanokporn, S. and Paiwan, S. (2021). Phytochemical screening, Antioxidant and sperm Viability of Nelumbo nucifera petal extract. MDPI, 10(7), 1375. [Crossref] https://doi.org/10.3390/plants10071375
Junaid, R. S. and Patil, M. K. (2020). Qualitative test for preliminary phytochemical screening; An Overview. International Journal of Chemical studies, 8(2), 603 - 608. [Crossref]
https://doi.org/10.22271/chemi.2020.v8.i2i.8834
Lancho, M. M., Getachew, M. D., Dagmawat, A. B., Edilu, J. S, Askale, G., Wakuma, M. B., Morka, D. B., Petros, A., Abraham, M., Miressa, T., Kebede, A., and Dejene, B. (2021). Phytochemical screening and in - Vitro evaluation of Antibacterial activities of Echinops amplexicaulis, Ruta Chalepenasis and salix subserrata against selected pathogenic bacterial strains in West Shewa zone, Ethiopia. Journal of Experimental pharmacol 13, 511 - 520. [Crossref] https://doi.org/10.2147/JEP.S305936
Leonard, O. A. Salifu, V. and Samuel, A. S. (2022). Dyestuff utilization of the bark of parkia biglobosa grown in Ankpa, Kogi, Nigeria. FUW Trend in science and technology jounal.7(2). 862 - 866.
Letiele, B. D., Amanda, L. G., Thainara, D. A., Thais, F. K., Jefferson, E., Rafael, R. M., Vanusa, M. and Elton, l. G. D. (2020). phytochemical analysis, Antioxidant Activity, Antimicrobial Activity and Cytotoxicity of Chaptalia Nutans leaves. Hindawii Advance in Pharmacological and pharmaceutical Science, [Crossref] https://doi.org/10.1155/2020/3260745
Mohammed, S. A. ans Aisha, A. Y. (2022). Comparative phytochemical screening and acute toxicity study of two varieties of ginger, zingber officinale. UMYU Scientifica. 1(1). 6 - 11. [Crossref]
https://doi.org/10.56919/usci.1122.002
Mohan, E., Suniya, S., Shanmugan, S. and Rajendran, K. (2021). Qualitative Phytochemical screening of selected medicinal plants. Journal of Drug Delivery and Therapeutic, 11(2), 141- 144. [Crossref] https://doi.org/10.22270/jddt.v11i2.4609
Muhamat, S. A., Lahat, N., Cyrille, A. N., Mady, C. and Mar, D. C. (2021). Phytochemical screening, Total Phenolic and Antioxidant activities of numerous extracts from leaves and bark of bauhinia rufescens lam. Food and Nutrition Science,12(6), 544 - 551. [Crossref]
https://doi.org/10.4236/fns.2021.126041
Nguyen, T. M., Dang, X. C., Pham, V. T., Trying, N. M., Thuc- Huy, D., Tran, T. L. M. and Vo, T. T. (2021). Phytochemical screening and Evaluation of Antioxidant properties and Antimicrobial activities against xanthomonas axonopodis of Euphorbia tricucalli extract in Binh Thuan Province, Vietnam. MDPI Molecules, doi.org/10:3390/Molecules. 26040941.
Nigam, R. and Armold, R. (2020). Qualitative and Quantitative Phytochemical screening and Clinical finger print Analysis of Herbal plant phyllanthus niruri using HPTLC. Journal of Scientific Research, 13(2), 623 - 633. [Crossref]
https://doi.org/10.3329/jsr.v13i2.50391
Nirmala, B.R., Sitakumari, O. and Rajesh, G. G. (2016). Phytochemical analysis and antimicrobial activity of Lawsonis inermis (Henna). Journal of plant science and research. 3(2). 158.
Olamide, E. A., Funsho, O. O., Tan, N., and Zeng, G. (2017). In Vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni - Suef university journal of Basic and Applied Sciences, 6(1), [Crossref]
https://doi.org/10.1016/j.bjbas.2016.12.003
Prashant, T., Bimlesh, K., Mandeep, K., Gurpreet, K. and Harleen, K. (2011). Phytochemical screening and Extraction: A review. International Pharmaceutical Sciencia, 1(1), 98 - 106.
Reddy, K. R. (1988). Folk medicine from Chittor district Andhra Prdesh India used in the treating of jaundice. International journal of crude drug research. 26. 137 - 140. [Crossref]
https://doi.org/10.3109/13880208809053907
Rojas-Sandovaj, J. (2017). Lawsonia inermis (Egyptian privet). Invasive species compendium. wallingford. Uk. CABI. [Crossref]
Shobha, P. R., Shanthi, V., and Rashed, M. A. (2015). Evaluation of Antioxidant activities of some medicinal plants using oxidizing agents in acidic medium by colorimetry. Int. J. Innov. Sci. Engr & Techn. 2(5). Retrieved from www.ijiset.com on 12/04/2022
Syede, N. F. (2018). Pharcognostic assessment of Lawsonia inermis flowers. Journal of pharmacognosy and phytochemistry. 7(6). 2365 - 2369
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 UMYU Scientifica
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
&
