Evaluation of Antibacterial Activity of Selected Medicinal Plant on Extended Spectrum β-lactamase Producing Salmonella enterica serovar Typhimurium
DOI:
https://doi.org/10.56919/usci.2541.003Keywords:
Salmonella enterica , Serovar Typhimurium, ESBL, antibacterial activity, medicinal plantAbstract
Study’s Excerpt:
- The resistance of ESBL-producing S. Typhimurium to antibiotics, urges need for plant-based alternatives.
- Blood samples of typhoid patients were analyzed with standard microbiological techniques.
- Typhi was detected in 79.5% of 200 samples using PCR techniques.
- Extracts of Brazilian tea, siam weed, neem, and ginger showed strong antibacterial effects.
- Plant-based remedies may combat resistant S. Typhi, advancing infectious disease treatment.
Full Abstract:
The growing prevalence of ESBL-producing Salmonella enterica serovar Typhimurium in typhoid fever patients, with resistance to most antibiotics, has heightened the need for plant-based remedies. This study was designed to evaluate the antibacterial activity of selected medicinal plants on ESBL-producing Salmonella enterica serovar Typhimurium from a clinical sample in Nigeria. A total of 200 blood samples were collected from patients suspected of typhoid fever and analyzed using standard microbiological techniques for isolation and characterization on the VITEK 2 system. Salmonella enterica serovar Typhimurium was screened for ESBL genes by Polymerase Chain Reaction specific primers. The antibacterial activity of ethanol extract from siam weed, bush cane, neem, Brazilian tea leaves, garlic, and ginger cloves was performed using the Agar well diffusion Technique. Of the 200 samples, S. Typhi accounted for 159(79.5 %). The amplification of the ESBL gene revealed a high proportion of CTX-M (n=159/100 %) followed by SHV (n=81/50.9 % and TEM (n=52/32.7 %). The results of the antibacterial activity of Brazilian tea, siam weed, neem, and ginger indicate that the extracts were effective at 100mg/ml, 50mg/ml, and 25mg/ml concentrations ranging from 100-46. 0%. Garlic had no antibacterial effect at 25mg/ml concentration, while All ESBL-producing Salmonella enterica serovar Typhimurium demonstrated resistance to bush cane extract across various concentrations. Since this study marks the first report of the antibacterial activity of these medicinal plant extracts on strain harboring such genotype, their prudent and judicious utilization is required in ethnobotanical/human medicine in treating ESBL-associated infectious diseases. There should be guidelines and regulations on the appropriate use of antibiotics to avert drug resistance and the spread of β-lactamase and other resistant determinants to susceptible strains.
References
Agbo O O, Thompson M D, Etu K E, NseAbasi P L, Elom K O, Peter I U, Iroha, I. R. Antibacterial activity of ethanol and methanol extract of Vernonia amygdalina, Ocimum gratissimum and Aloe barbadensis against Pseudomonas aeruginosa and Staphylococcus aureus Isolated from Wound Infected Patients. International Journal of Pharmacog and Pharm Res. 2024; 6(1): 54-60. https://doi.org/10.33545/26647168.2024.v6.i1a.69
Akhter R, Sarker M W. Antimicrobial activity in leaf extract of neem in broiler. Res Agric Livestock and Fisheries. 2019; 6:337–343. https://doi.org/10.3329/ralf.v6i2.43063
Akinyemi KO, AIwalokun B, Alafe OO, Mudashiru S A, Fakorede S. blaCTMgroup extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria. Infection and Drug Resistance. 2015; 8 99–106. https://doi.org/10.2147/IDR.S78876
Akinyemi, K O, Al-Khafaji NSK, Al-Alaq FT, Fakorede CO, Al-Dahmoshi HOM, Iwalokun BA, Akpabio I, Alkafaas SA, Saki M. Extended-spectrum Beta-lactamases Encoding Genes among Salmonella Enterica serovar Typhi Isolates in Patients with Typhoid Fever from four Academic Medical Centers in Lagos, Nigeria. Rev Invest Clin. 2022;74(3):165-71. https://doi.org/10.24875/RIC.22000078
Akpan M M, Odeomena C S, Nwachukwu C N, Danladi B. Antimicrobial assessment of ethanolic extract of Costus afer leaves Asian J Plant Sci Res. 2012; 2(3):335–341
Al Saiqali M, Tangutur A D, Banoth C, Bhukya B. Antimicrobial and Anticancer Potential of Low Molecular Weight Polypeptides Extracted and Characterized from Leaves of Azadirachta indica. Int J Biol Macromol. 2018; 114, 906–921. https://doi.org/10.1016/j.ijbiomac.2018.03.169
Ali E, Islam S, Hossen I, Khatun M, Islam A. Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multi-drug resistant pathogenic bacteria of poultry. Vet Med Sci. 2021; 7(5):1921–1927. https://doi.org/10.1002/vms3.511
Amadi P U, Agomuo E N, Ukaga C N, Njoku U C, Amadi J A, Nwaekpe C G. Preclinical Trial of Traditional Plant Remedies for the Treatment of Complications of Gestational Malaria. Med (Basel). 2021; 8 (12):34-56. https://doi.org/10.3390/medicines8120079
Ansary J, Forbes-Hernández TY, Gil E, Cianciosi D, Zhang J, Elexpuru-Zabaleta M, Simal-Gandara J, Giampieri F, Battino M. Potential Health Benefit of Garlic Based on Human Intervention Studies: A Brief Overview. Antioxidants 2020; 9: 619-620. https://doi.org/10.3390/antiox9070619
Arora S, Saquib S A, Algarni Y A, Kader M A, Ahmad I, Alshahrani M Y et al. Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study. Antibiotic. 2021; 10 (5):45-78. https://doi.org/10.3390/antibiotics10050552
Awol RN, Reda DY, Gidebo DD. Prevalence of Salmonella enterica serovar Typhi infection, its associated factors and antimicrobial susceptibility patterns among febrile patients at Adare general hospital, Hawassa, southern Ethiopia. BMC Infect Dis. 2021;21:30. https://doi.org/10.1186/s12879-020-05726-9
Bakeri SA, Yasin RM, Koh YT, Puthucheary SD, Thong, KL. Genetic Diversity of Human Isolates of Salmonella enterica Serovar Enteritidis. Malaysia J Appl Microbiol. 2003; 95, 773-780. https://doi.org/10.1046/j.1365-2672.2003.02033.x
Basnyat B, Qamar FN, Rupali P, Ahmed T, Parry CM. Enteric fever. BMJ 2021; 372-437. https://doi.org/10.1136/bmj.n437
Belguith H, Kthiri F, Chati A, Sofah AA, Hamida JB, Landoulsi A. Study of the effect of aqueous garlic extract (Allium Sativum) one some Salmonella serovars isolates. Emir J Food Agric. 2010; 22:189–206. https://doi.org/10.9755/ejfa.v22i3.4889
Bhatwalkar SB, Mondal R, Krishna SBN, Adam JK, Govender P, Anupam R. Antibacterial Properties of Organosulfur Compounds of Garlic (Allium sativum). Front Microbiol. 2021:12(61):30-77. https://doi.org/10.3389/fmicb.2021.613077
Boison D, Adinortey CA, Babanyinah G K, Quasie O, Agbeko R, Wiabo-Asabil G K, Adinortey M B. Costus afer: A Systematic Review of Evidence-Based Data in support of Its Medicinal Relevance. Scientifica. 2019; 37(3):2-68. https://doi.org/10.1155/2019/3732687
Braga T M, Rocha L, Chung T Y, Oliveira R F, Pinho C, Oliveira A I et al. Biological Activities of Gedunin-A Limonoid from the Meliaceae Family. Molecules. 2020; 25(3):103-390. https://doi.org/10.3390/molecules25030493
Choo S, Chin VK, Wong EH, Madhavan P, Tay ST, Yong PVC, Chong PP. Review: Antimicrobial properties of allicin Used alone or in combination with other medications. Folia Microbiol. 2020; 65:451–465. https://doi.org/10.1007/s12223-020-00786-5
Circella E, Casalino G, D’Amico F, Pugliese N, Dimuccio MM, Camarda A, Bozzo G. In Vitro Antimicrobial Effectiveness Tests Using Garlic (Allium sativum)Against Salmonella enterica Subspecies Enterica Serovar Enteritidis. Antibiotics. 2022; 11:14-81. https://doi.org/10.3390/antibiotics11111481
Djeffal S, Bakour S, Mamache B, Elgroud R, Agabou A, Chabou S, Hireche S, Bouaziz O, Rahal K, Rolain J. Prevalence and clonal relationship of ESBL producing Salmonella strains from humans and poultry in northeastern Algeria. BMC Veterinary Res. 2017; 13:132-138. https://doi.org/10.1186/s12917-017-1050-3
Dor Z, Shnaiderman-Torban A, Kondratyeva K, Davidovich-Cohen M, Rokney A, Steinman A, Navon-Venezia S. Emergence and Spread of Different ESBL-Producing Salmonella enterica Serovars in Hospitalized Horses Sharing a Highly Transferable IncM2 CTX-M-3-Encoding Plasmid Front Microbiol. 2020; 11:60-61. https://doi.org/10.3389/fmicb.2020.616032
Egwu E, Ibiam FA, Moses IB, Iroha CS, Orji I, Okafor-Alu FN, Eze CO, Iroha IR. Antimicrobial susceptibility and molecular characteristics of beta-lactam- and fluoroquinolone-resistant E coli from human clinical samples in Nigeria. Scientific African. 2023; 21:18-63. https://doi.org/10.1016/j.sciaf.2023.e01863
Felicia A, Debora K, Ramadhani R. In Vitro Antimicrobial Activity Evaluation of Ginger (Zingiber officinale) Absolute Ethanol Extract against Uropathogenic Escherichia coli (UPEC). Journal Ilmiah Mahasiswa Kedokteran Universitas Airlangga. 2022; 13(02):52-53. https://doi.org/10.20473/juxta.V13I22022.51-56
Gberikon GM, Aguoru CU, Ogbonna, IO. ESBL Production and Multidrug Resistance of Salmonella Serovars Isolates in Benue State. Am J Mol Biol. 2020; 10, 200-223. https://doi.org/10.4236/ajmb.2020.103014
Gull I, Saeed M, Shaukat H et al. Inhibitory Effect of Allium Sativum and Zingiber Officinale Extracts on Clinically Important Drug Resistant Pathogenic Bacteria. Ann Clin Microbiol Antimicrob. 2012; 11:8-23. https://doi.org/10.1186/1476-0711-11-8
Gupta S C, Prasad S, Tyagi A K, Kunnumakkara A B, Aggarwal B B. Neem (Azadirachta indica): An Indian Traditional Panacea with Modern Molecular Basis. Phytomedicine. 2017; 34:14–20. https://doi.org/10.1016/j.phymed.2017.07.001
Husna A, Rahman MM, Badruzzaman A T M, Sikder M H, Islam M R, Rahman M T, Alam J, Ashour H M. Extended-Spectrum β-Lactamases (ESBL): Challenges and Opportunities. Biomedicines. 2023; 11:2937-2939. https://doi.org/10.3390/biomedicines11112937
Ibrahim N, Kebede A. In Vitro antibacterial Activities of Methanol and Aqueous Leave Extracts of Selected Medicinal Plants against Human Pathogenic Bacteria. Saudi J Biol Sci. 2020; 27 (9), 2261–2268. https://doi.org/10.1016/j.sjbs.2020.06.047
Ibrahim T, Ngwai YB, Ishaleku D, Tsakub PA, Nkene IH, Abimiku RH. Detection of extended spectrum beta-lactamase (ESBL)–production in Salmonella Typhimurium isolated from poultry birds in Nasarawa State, Nigeria. Scientific African. 2022;16:12-43. https://doi.org/10.1016/j.sciaf.2022.e01243
Izah S C, Etim N G, Ilerhunmwuwa I A, Ibibo T B, Udumo J J. Activities of express extracts of costus afer Ker–Gawl [Family COSTACEAE] against selected bacterial isolates, Int J pharm Phytopharm Res. 2019; 9(4):39-44
Jagannathan J, Nagar P, Kaniappan A S, Raveendran A, Shekhar S. Comparison of Antimicrobial Efficacy of Natural Extracts as a Disinfectant for Removable Orthodontic Appliances: An Ex Vivo Study Int J Clin Pediatr Dent. 2020; 13(6):640–643. https://doi.org/10.5005/jp-journals-10005-1850
Jajere SM. A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multi-drug resistance. Vet World 2019;12:504-21. https://doi.org/10.14202/vetworld.2019.504-521
Jesus M, Martins A P, Gallardo E, Silvestre S. Diosgenin: recent highlights on pharmacology and analytical methodology. J Anal Meth Chemistry. 2016; 16-20. https://doi.org/10.1155/2016/4156293
José MFB, Machado RP, Araujo PAB, Speretta GF. Physiological effects of yerba maté (Ilex paraguariensis): A systematic review. Nutr Rev. 2023; 81(9):1163-1179. https://doi.org/10.1093/nutrit/nuac109
Joseph I S, Okolo I O, Udenweze E C, Nwankwo C E, Peter I U, Ogbonna I P, Iroha I R. Comparison of Antibiotic-Resistant Pattern of Extended Spectrum Beta-Lactamase and Carbapenem- Resistant Escherichia coli Isolates from Clinical and Non-Clinical Sources, Journal of Drug Delivery and Therapeutics. 2023; 13(7):107-118. https://doi.org/10.22270/jddt.v13i7.5918
Kanth M R, Prakash A R, Sreenath G, Reddy V S, Huldah S. Efficacy of Specific Plant Products on Microorganisms Causing Dental Caries J Clin Diagn Res. 2016;10 (12):1–3. https://doi.org/10.7860/JCDR/2016/19772.9025
Kaur R, Tiwari A, Manish M, Maurya I K, Bhatnagar R, Singh S. Common Garlic (Allium Sativum L) Has Potent Anti-Bacillus Anthracis Activity. J Ethnopharmacol. 2021; 264(11):32-30. https://doi.org/10.1016/j.jep.2020.113230
Kela E, Sogbesan AO, Wakil UB. Evaluation of Phytochemical Composition of Ginger Extracts. Fish Aqua J. 2023; 14:317-319.
Lutomski P, Goździewska M, Florek-Łuszczki M. Health properties of yerba mate. Ann Agric Environ Med. 2020; 27(2):310-313. https://doi.org/10.26444/aaem/119994
Mather AE, Reid SWJ, Maskel DJ, Parkhill J, Fookes MC, Harris SR, Brown DJ, Coia JE, Mulvey MR, Gilmour MW Petrovska L, de Pinna E, Kuroda M, Akiba M, Izumiya H, Connor TR, Suchard MA, Lemey P, Mellor DJ, Haydon, DT, Thomson NR. Distinguishable Epidemics of Multidrug-Resistant Salmonella Typhimurium DT104 in Different Hosts. Science. 2013; 341, 1514-1517. https://doi.org/10.1126/science.1240578
Nadimpalli M, Fabre L, Yith V, Sem N, Gouali M, Delarocque E et al. CTX-M 55-type ESBL-producing Salmonella enterica are emerging among retail meats in Phnom Penh, Cambodia. J Antimicrob Chemother 2019; 74: 342–348. https://doi.org/10.1093/jac/dky451
Nagini S, Nivetha R, Palrasu M, Mishra R. Nimbolide, a Neem Limonoid, Is a Promising Candidate for the Anticancer Drug Arsenal. J Med Chem. 2021; 64 (7):3560–3577. https://doi.org/10.1021/acs.jmedchem.0c02239
Nakamoto M, Kunimura K, Suzuki JI, Kodera Y. Antimicrobial properties of hydrophobic compounds in garlic: Allicin, Vinyldithiin, ajoene and diallyl polysulfides. Exp Ther Med. 2020; 19:1550–1553. https://doi.org/10.3892/etm.2019.8388
Nwankwo CH, Okolo I, Mba A, Uzoeto H, Udenweze E, Okoli F, Thompson M, Chukwu E, Bassey N, Ngwu J Peter I In Vitro Assessment of Conventional and Plant-derived Antifungal Agents against Candida Species Prevalence among Pregnant Women in Southeastern Nigeria. Int J Pharm Phytopharmacol Res. 2023;13(1):13-8. https://doi.org/10.51847/64mXiTGGOe
Nwode, V.F., Akindele, K., Edemekong, C. I., Ugwu, U. N., Asumpta, F. C., Obya, I. M., Nwatu, A. N., Onu, H., Peter, I. U., Iroha, I. R (2024). Antibacterial Activities of Herbal Extracts and Conventional Antibiotics on Methicillin Resistant Staphylococcus aureus Isolated from Wound. European Journal of Science, Innovation and Technology, 4(4):45-56.
Nwosu, U. O., Ibiam, F. A., Amadi-Ibiam, C.O., Iroha, C. S., Edemekong, C. I., Peter I. U and Iroha, I. R (2023). Fecal carriage of extended spectrum beta-lactamase and fluoroquinolone resistant gene in non-typhoidal Salmonella enterica isolates from food producing animals and humans. Journal of Drug Delivery and Therapeutics, 13(9):128-134. https://doi.org/10.22270/jddt.v13i9.5964
Okpa BO, Gberikon GM, Aguoru CU, Ogbonna IO.ESBL Production and Multidrug Resistance of Salmonella Serovars Isolates in Benue State Am J Mol Biol. 2020; 10: 200-223. https://doi.org/10.4236/ajmb.2020.103014
Onyenwe N E, Nnamani N D, Okoro JC, Nwofor C N, Jesumirhewe C. Prevalence and gene sequencing of extended spectrum β-lactamases producing Salmonella enterica serovar Typhi from South-East Nigeria Afri J Pharm Pharmacol. 2020; 14(7):192-202. https://doi.org/10.5897/AJPP2020.5115
Pereira NM, Shah I. Cephalosporin-resistant typhoid. SAGE Open Med Case Rep 2020;8:205-313. https://doi.org/10.1177/2050313X20917835
Peter IU, Edemekong C, Balogun OM, Ikusika BA, Ngwu JN, Bassey NU, Mohammed ID, et al Phytochemical Screening and Antibacterial Activity of Aqueous Extract of Costus afer Plant on Selected Multidrug-Resistant Bacteria. IOSR J Pharmacy and Biol Sciences 2022; 17(1):39-42
Peter, I.U., Ogbonna, I.P., Edemekong, C. I., Okolo, I. O and Mohammed, I. D (2024). Screening for genes Encoding Virulence Factor in Salmonella serovar Typhimurium isolated from Tiger nut Juice. Ibom Medical Journal, 17(3): 393 - 400
Popa GL, Papa MI. Salmonella spp infection-a continuous threat. Worldwide Germs. 2021;11:88-96. https://doi.org/10.18683/germs.2021.1244
Primeau CA, Bharat A, Janecko N, Carson CA, Mulvey M, Reid-Smith R, McEwen S, McWhirter JE, Parmley EJ. Integrated surveillance of extended-spectrum beta-lactamase (ESBL)-producing Salmonella and Escherichia coli from humans and animal species raised for human consumption in Canada from 2012 to 2017. Epidemiology and Infection. 2023; 151(14) 1–11. https://doi.org/10.1017/S0950268822001509
Quesada I, de Paola M, Torres-Palazzolo C, Camargo A, Ferder L, Manucha W, Castro C. Effect of garlic’s active Constituents in inflammation, obesity and cardiovascular disease. Curr Hypertens Rep. 2020; 22-6. https://doi.org/10.1007/s11906-019-1009-9
Robinson K, Assumpcao ALFV, Arsi K, Donoghue A, Jesudhasan PRR. Ability of Garlic and Ginger Oil to Reduce Salmonella In Post-Harvest Poultry. Animals. 2022;12:29-74. https://doi.org/10.3390/ani12212974
Rotimi V O, Jamal W, Pal T, Sovenned A, Albert MJ. Emergence of CTX-M-15 Type Extended-spectrum -lactamase-producing Salmonella species in Kuwait and the United Arab Emirates. J Med Microbiol. 2008; 57:881-886. https://doi.org/10.1099/jmm.0.47509-0
Saeed M, Rasool MH, Rasheed F, Saqalein M, Nisar MA, Imran AA, et al. Extended-spectrum beta lactamases producing extensively drug-resistant Salmonella Typhi in Punjab, Pakistan. J Infect Dev Ctries. 2020;14:169-76. https://doi.org/10.3855/jidc.12049
Saleem S, Muhammad G, Hussain M A, Bukhari S N A. A Comprehensive Review of Phytochemical Profile, Bioactives for Pharmaceuticals, and Pharmacological Attributes of Azadirachta indica. Phytother Res. 2018;32 (7):1241–1272. https://doi.org/10.1002/ptr.6076
Saliu EM, Vahjen W, Zentek J. Types and prevalence of extended–spectrum beta–lactamase producing Enterobacteriaceae in poultry. Animal Health Res Rev. 2017; 18(1): 46–57. https://doi.org/10.1017/S1466252317000020
Thakor HJ, Rathi Y S, Nayak N S. Phytochemical Screening of Ginger (Zingiber Officinale), a Medicinal Plant. Sch Int J Tradit Complement Med. 2023; 6(4): 58-62. https://doi.org/10.36348/sijtcm.2023.v06i04.002
Thung TY, Radu S, Mahyudin NA, Rukayadi Y, Zakaria Z, Mazlan N. Prevalence, virulence genes and antimicrobial resistance profiles of Salmonella serovars from retail beef in Selangor, Malaysia. Front in microbiol, 2018; 8:26-97. https://doi.org/10.3389/fmicb.2017.02697
Worku W, Desta M, Menjetta T. High prevalence and antimicrobial susceptibility pattern of Salmonella species and extended spectrum β-lactamase producing Escherichia coli from raw cattle meat at butcher houses in Hawassa city, Sidama regional state, Ethiopia. PLoS ONE. 2022; 17(1): 262-308. https://doi.org/10.1371/journal.pone.0262308
Wu H, Xia X, Cui Y, Hu Y, Xi M, Wang X, Yang B. Prevalence of extended- spectrum β-lactamase-producing Salmonella on retail chicken in six provinces and two national cities in the People’s Republic of China. J Food Prot. 2013; 76:2040–2044. https://doi.org/10.4315/0362-028X.JFP-13-224
Wylie MR, Merrell DS. The Antimicrobial Potential of the Neem Tree Azadirachta indica Front Pharmacol. 2022; 13: 891-535. https://doi.org/10.3389/fphar.2022.891535
Zihadi M A H, Rahman M, Talukder S, Hasan M M, Nahar S, Sikder M H. Antibacterial Efficacy of Ethanolic Extract of Camellia Sinensis and Azadirachta indica Leaves on Methicillin-Resistant Staphylococcus aureus and Shiga-Toxigenic Escherichia coli. J Adv Vet Anim Res. 2019; 6 (2): 247–252. https://doi.org/10.5455/javar.2019.f340
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Copyright (c) 2025 Christiana Inuaesiet Edemekong, Anulika Goodness Chukwujekwu, Maria Ezinne Okorie, Evangeline Udenweze, Ijeoma Frederica Obodoechi, Ifeoma Precious Ogbonna, NseAbasi Peter Livinus
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