Histopathological and Oxidative Stress Response in African Catfish Clarias gariepinus in Heavy Metal Contaminated Water from the Hadejia-Nguru Wetland North Eastern Nigeria.
DOI:
https://doi.org/10.56919/usci.1222.010Keywords:
clariid fish, antioxidants, toxicity, freshwater wetlands, pollutantsAbstract
The Hadejia-Nguru wetland is a source of drinking, farming, and natural fertilization of fields, fishing and transportation. Discharges from agricultural activities, sewage and chemical application find their way into this water body. The present study is aimed at evaluating levels of some heavy metals (Hg, Pb, Cd, Cr and Al) in tissues of Clarias gariepinus (gills, liver and muscles) collected at 5 sampling sites labelled as A-E. Histopathological examination and the presence of antioxidant enzymes revealed the extent of damage in tissue and stress in the fish. Results of the heavy metal reveals concentrations in the sequence Pb>Cr>Hg>Al>Cd revealing a concentration higher than the permissible maximum residue limit as recommended by FAO and WHO. The activities of superoxide dismutase (SOD) catalase (CAT), malondialdehyde (MDA) and reduced glutathione were seen in elevated levels in the gills, liver and muscles. The highest level of SOD was found in the liver with a mean concentration of 32.43u/ml followed by a concentration level 12.35u/ml in the gills, CAT levels was highest in the liver with a concentration level of 67.80u/ml, MDA was highest in the gills with a concentration of 9.06n/mol and there was no significant difference (P<0.05) between concentration of MDA in the liver with other organs. GSH levels was highest in the gills with a concentration of 1016.64µg/ml and there was a significant difference (P<0.005) between the concentration of GSH in the gills in comparison to other organs. Histopathology revealed different deleterious effects in the gill filaments, hepatocytes and bowman’s space in the liver and muscle cells respectivly. The presence of metal toxicity, antioxidant enzymes and tissue disorders in fish are indication of pollution and can serve as bio-monitoring model of the safety of fresh water bodies.
References
Akindele, O.A.et al (2012). Rat model of food-induced non-obese-type 2 diabetes mellitus; comparativepathophysology and histopathology. Int J Physiol Pathophysiol Pharmacol,4(1): p. 51-58.
Allain, C.C., Poon, L.S., Chan, C.S.G., Richmond, W. & Fu, P.C. (1974). Enzymatic determination of total serum cholesterol: Clinical chemistry, 20, 470. https://doi.org/10.1093/clinchem/20.4.470
Barham, D., Trinder, P. (1972). Quoted in Cheesbrough: Medical laboratory manual for tropical countries, Vol I (2nded).
Basiru, O. A., Babatunji, E. O., Precious, E. A. and Oluwafemi, A. O. (2022). Hepatoprotective and Haematoprotective Roles of Gongronema latifolium Benth Aqueous Extract in AlloxanInduced Diabetic Rats. Biointerface Research In Applied Chemistry. Volume 12, Issue 1, 537 - 546. https://doi.org/10.33263/BRIAC121.537546
Burstein, M., Scholnick, H.R., Morfin, R. (1970). Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions, J Lipid Res, 11, 583-595. https://doi.org/10.1016/S0022-2275(20)42943-8
Chaney, A.L., Marbach, A.L., (1962). Clin. Chem; 8: 130edition) ELBS, Cambrdige, pp. 527-455.
https://doi.org/10.1093/clinchem/8.2.130
Chase, S.L., New lipid guidelines recommend tighter control. Adv Practice Nursing e-J, 2002; 2: 1-9
Evans WC (2002). Saponins, Cardioactive drugs and other steroids. In:
Trease and Evans Pharmacognosy, 15th Edition.
Fawcett, J.K., Scott, J.E. (1960). Journal of Clinical Pathology. 13: 156. https://doi.org/10.1136/jcp.13.2.156
Friedewald, W.T., Levy, R.I., Fredrickson, D.S. (1972). Estimation of LDL-C in plasma without the use of the preparative ultracentrifuge, Clinical Chemistry, 35, 1721-1728.
Gupta, A.K and Jain, A. (2019). Phyto-chemical and therapeutic briefing of Kigelia africana (Lam.) Benth. Indian J Pharm Biol Res. 7(01):14-22. https://doi.org/10.30750/ijpbr.7.1.4
Harris, D. C. (1995). Quantitative Chemical Analysis. W. H. Freeman and Company, New York. Chapter21
Hassan, S.W., Abubakar, M.G., Umar, R.A., Yakubu, A.S., Maishanu, H.M. and Ayeni, G., (2011). Pharmacological and toxicological properties of leaf extracts of Kigelia africana. Journal of Pharmacology and Toxicology, 6(2), 124-132. https://doi.org/10.3923/jpt.2011.124.13
Hauwa'u, Y. B., Ja'afaru, S. M., Peter, M. W., Timothy, B., Mohammad, Y. G. and Maimuna, M. Z. Lipid Profile Of Alloxan-Induced Diabetic Wistar Rats Treated With Methanolic Extract Of Adansonia Digitata Fruit Pulp. Science World Journal Vol 9 (No 2) 2014. ISSN 1597-6343.
Johnson, M., Longe, A.O., Campbell, C.A., Omotayo, M.A. (2014). Evaluation of Antidiabetic And The Effect Of Methanolic Leaf Extract Of Jatropha Curcas On Some Biochemical Parameters In Alloxaninduced Diabetic Male Albino Rats. European Journal of Medicinal Plants: 1501-1512. https://doi.org/10.9734/EJMP/2014/12500
K.O, Alsaad and A.M. (2007). Herzenberg, Distinguishing diabetic nephropathy from other causes of glomerulosclerosis: an update, J. Clin. Pathol. 60 (1) 18-26. https://doi.org/10.1136/jcp.2005.035592
Kenkel, J. (2003). Analytical Chemistry for Technicians. CRC Press LLC. Boca Raton. Pp. 108-109. https://doi.org/10.1201/9781420056709
Kumar, S., Kumar, V. and Prakash, O. (2011). Antidiabetic and hypolipidemic activities of
Dillenia indica extract in diabetic rats. (5):570-574.
Lorke, U. C. (1983). Determination of Lethal Dose of xeno-biotics in experimental animals. Nature 45: 264-266.
L.J. Vleming, J.J. Baelde, R.G. Westendorp, M.R. Daha, L.A. van Es, J.A. Bruijn, The glomerular deposition of PAS positive material correlates with renal function in human kidney diseases, Clin. Nephrol. 47 (3) (1997 Mar) 158-167. PMID: 9105762.
Obianagha, N. F., Okafor, C. J., Chukwuani, U., Obeagu, E. I., Ogundahunsi, O. A., Ogbonnia, S. O., Yusuf, S. A. and Galano, E. S.. (2021). Effect of Extracts of Kigelia Africana Fruit and Sorghum Bicolor Stalk on the Biochemical Parameters of Alloxan-Induced Diabetic Rats. Journal of Pharmaceutical Research International 33(25B): 86-97, 2021; Article no.JPRI.67624 ISSN: 2456-9119.
https://doi.org/10.9734/jpri/2021/v33i25B31465
Njoku OV, Obi C. Phytochemical constituents of some selected medicinal plants. African Journal of Pure and Applied Chemistry. 2009; 3(11):228-233
Oduola, T., Adeniyi, F.A., Ogunyemi, E.O., Idowu, T.O. and Subair, H.G. (2007). Toxicity studies on an unripe Carica papaya aqueous extract; Biochemical and hematological effects in wistar albino rats. J. Med. Plants Res., 1: Pp. 1-4.
Ogbonnia S, Mbaka G, Akinsande O, Otah D, Ayeni T. (2016). Evaluation of acute toxicity and hypoglycaemic and hypolipidaemic effectsof Cyathulaprostata (Linn.) blume weeds on adult rats. Br J Pharm Res. 9(5): https://doi.org/10.9734/BJPR/2016/20433
Ojo, O.A., Ojo, A.B., Ajiboye, B.O., Imiere, O., Oyinloye, B.E. (2020) Antihyperlipidemic activities and hematological potentials of ethanol extract of Blighiasapida Koenig bark in alloxan-induced diabetic rats. Serbian Journal of Experimental and Clinical Research, 21, 11-17, https://doi.org/10.2478/sjecr-2018- 0042.
Reitman, S., Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxaloacetate and glutamic pyruvic transaminases. Am J ClinPathol;28:5661
https://doi.org/10.1093/ajcp/28.1.56
S. W. Hassan , A. N. Ukwuani-Kwaja , U. D. Nuhu and R. D. (2020). Jabaka. Acute and Subchronic Toxicity Studies of Combretum collinum Methanol Root Extract in Albino Rats. International Journal of Biochemistry Research & Review 29(10): 9-28, Article no.IJBCRR.64246 ISSN: 2231-086X, NLM ID: 101654445. https://doi.org/10.9734/ijbcrr/2020/v29i1030235
Sa"id S.S., et al., (2019). "Effects of White Grub Extracts On Serum Glucose and Lipid Profile of Alloxan Induced Diabetes in Rats". Annals of Biological Research, 10(10): 21-29
Said, S.S., Dambazau, S.M. and Abdullahi, M.A., (2021).Antibacterial and Toxicity Evaluation of Stem Bark Extract of Kigelia africana (Lam.) Benth, Glob Acad J Agri Biosci; 3(3).
Singh V, Kumar R. (2017). Study of Phytochemical Analysis and Antioxidant Activity of Allium sativum of Bundelkhand Region. International Journal of Life Sciences Scientific Research. 3(6):1451-1458. https://doi.org/10.21276/ijlssr.2017.3.6.4
Sushruta, K, Satyanarayana S, Srinivas S, Sekhar, J. R. (2007). Evaluation of the blood glucose reducing effects of aqueous extracts of the selected umbelliferous fruits used in culinary practices. Trop J Pharm Res. 5(2):613-7. https://doi.org/10.4314/tjpr.v5i2.14639
Trinder, P. (1969). Cholesterol enzymatic end point manual. Ann clinBiochem, 6, pp. 24-25.
https://doi.org/10.1177/000456326900600505
Thomas, M.C., MacIsaac, R. J., Tsalamandris, C., Power, D. and Jerums, G. (2003). Unrecognized anemia in patients with diabetes: a cross-sectional survey, Diabetes Care. 26 (4) 1164-1169.
https://doi.org/10.2337/diacare.26.4.1164
Uhuo, E. N, Ezeanyika, L.U.S & Ogugua, V.N. (2019). Oxidative and Biochemical Parameters Analysis of Alloxan-Induced Diabetic Rats Administered Methanol Leaf and Fruit Extracts of Kiglia africana. London Journal of Research in Science: Natural and Formal.
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